Real skin, Real Insights: Evaluating Biodistribution and Wound Healing of Cell-free Amniotic Fluid with an ex vivo Viable Human Skin Model

This white paper explores how cell-free amniotic fluid (cfAF) distributes within human skin and its impact on wound repair using a physiologically relevant ex vivo human skin model. By combining advanced analytical techniques with a translational tissue platform, the study provides critical insight into both mechanism of action and therapeutic potential.

Key Findings:

• Ex vivo human skin models provide a predictive platform for evaluating complex biologics
• cfAF treatments reduced wound area over time indicating enhanced wound healing in ex vivo skin
• Biodistribution of cfAF can be directly correlated with wound healing outcomes
• cfAF MTX-00 is a promising biologic under development for wound care
• LC-MS/MS enables sensitive detection of low-abundance proteins in complex sample matrices with high sensitivity (pg of total protein), specificity, and resolution