Histology & Imaging

At iFyber, we have access to state-of-the-art equipment and facilities that allow our highly trained staff to process and stain a variety of tissue types for research and development purposes. We specialize in working with clients across the medical device, wound care, and regenerative medicine fields to customize histological techniques for signal quantification or morphological measurements in a way that best fits their testing and data needs.

We offer a variety of routine and advanced histological methods that can be customized for a range of tissue types, including:

  • Light microscopy
  • Wet mount preparation
  • Tissue fixation, processing, embedding, sectioning, and mounting
  • Slide scanning:  GLP and non-GLP
  • Routine hematoxylin and eosin (H&E) staining and special staining (e.g., periodic acid-Schiff [PAS], Masson’s trichrome, Nissl staining)
  • Simple staining for bacteria (e.g., methylene blue, safranin, crystal violet)
  • Differential staining (e.g., Gram staining, GMS staining)
  • Immunohistochemistry (IHC)
  • In situ hybridization (ISH)
  • LIVE/DEAD staining
  • Confocal microscopy and 3D reconstruction
    • Visualization of bacteria using GFP or luciferase (lux) reporter systems
  • Image processing and morphology measurements

As a complement to our antimicrobial offerings, iFyber routinely performs histological analyses and microscopy techniques for evaluating biofilm prevention and eradication using our ex vivo model of mature biofilm. Visualization of bacteria can be performed through LIVE/DEAD staining or fluorescence or luminescence (e.g., bacterial GFP or luciferase [lux] reporter systems). These techniques allow for qualitative visual evidence of the effects of anti-biofilm treatments on the structure and morphology of underlying tissues and the prevention or eradication of infiltrating biofilm. Data derived from these models can aid the development of effective device- and drug-based wound care treatments. iFyber offers GLP-compliant testing services.

Contact us to learn more about iFyber’s histology offerings.

iFyber has adopted an ex vivo porcine dermal model of mature biofilm as a bridge between standard in vitro antimicrobial and anti-biofilm assays and in vivo animal studies. This ex vivo model uses porcine dermal explants with artificial wound beds that support biofilm populations closely resembling those seen in human chronic wounds. This model is suitable for evaluation of both biofilm prevention and eradication and can be used to assess topical antibiotic treatment efficacy for wound care applications. Treatment results using this model are typically quantified through recovery and enumeration of
both total and biofilm-associated bacteria remaining on the explant; however, biofilm formation in the wound bed can be also evaluated by histological analysis and confocal microscopy. These techniques allow for qualitative visual evidence of the effects of anti-biofilm treatments on the structure and morphology of underlying tissues and the prevention or eradication of the biofilm itself.