Liquid Chromatography Mass Spectrometry (LC-MS) Analysis
Proteomics, Metabolomics, E&L Testing, Environmental Contaminants, and Beyond
Liquid Chromatography–Mass Spectrometry (LC-MS) has revolutionized how researchers, product developers, and innovators answer the essential question: “What’s in this sample?”
At iFyber, our LC-MS services provide more than data. We deliver deep insight through custom method development, high-resolution analysis, and expert interpretation across complex biological, environmental, and material samples.
Across pharma, biotech, medical device, and academic sectors, teams rely on untargeted and discovery-phase LC-MS to investigate unknowns, relieve capacity bottlenecks, and generate meaningful biological or mechanistic insights. Our workflows are built for lean teams, busy core facilities, and exploratory R&D groups that need fast, clear answers.
What sets iFyber apart is how we integrate LC-MS with our broader R&D capabilities including microbiology, molecular and cellular biology, polymer and biomaterial characterization, bioanalytical chemistry, and in vitro/ex vivo models. This allows us to design studies that not only identify what’s present, but also explain its biological relevance, material performance, or regulatory implications.
From medical device development to therapeutic innovation, iFyber’s LC-MS services provide the clarity and context needed to move research forward with confidence.
LC-MS Areas of Focus
- Untargeted Discovery Proteomics & Chemical Profiling: For exploratory, mechanism-driven, or “we don’t know what’s in the sample” scenarios. This includes discovery proteomics, unexpected analytical results, unknown compound ID, and systems-level biological analysis.
- Targeted Quantitative Analysis: For validated compounds, biomarkers, known analytes, or structured E&L workflows. Ideal for confirming expected results or quantifying known targets during method development or product evaluation.
- Overflow Analytical Capacity: Built for teams facing instrument backlogs or limited internal mass spectrometry staffing including pharma, biotech, and academic cores. We align our workflows to yours for seamless handoff and reproducible data.
LC-MS Instrumentation
iFyber’s LC–MS services are powered by the Orbitrap Excedion Pro Hybrid Mass Spectrometer, a state-of-the-art system that delivers enhanced sensitivity, high resolution, and dynamic flexibility required for today’s complex analytical challenges.
This advanced platform combines high-resolution mass analysis, electron-based fragmentation, and gas-phase fractionation to capture molecular information with exceptional clarity. Designed for biopharma, metabolomics, proteomics, and structural biology applications, this platform sets a new standard in analytical performance, empowering groundbreaking discoveries.
Key features and Technology:
iFyber’s LC-MS platform combines cutting-edge mass spectrometry, ionization, and chromatography technologies to deliver high-resolution, high-sensitivity analysis for complex biological, environmental, and material samples.
By combining these technologies, iFyber delivers high-sensitivity liquid chromatography mass spectrometry analysis that supports both exploratory discovery and targeted quantification helping researchers and product developers uncover meaningful insights across biological, environmental, and material systems.
- Ultra-High-Field Orbitrap Mass Analyzer – Up to 70 Hz MS² scans for fast, precise data acquisition.
- Quadrupole & Ion-Routing Multipole – Enables versatile fragmentation strategies.
- EASY-IC & ETD – Accurate mass calibration and electron-based fragmentation for detailed molecular characterization.
- Bent Trap – High-duty cycles for enhanced sensitivity.
- FAIMS Pro Duo Interface – Online gas-phase fractionation reduces noise and improves system robustness.
- OptaMax™ Plus Ion Source & EasySpray™ – Ideal technolgy for high-sensitivity LC-MS data.
- Seamless Thermo Scientific™ PepMap™ Neo Column Integration – tool-free setup, superior separation performance, and column-to-column reproducibility, enhancing overall workflow flexibility.
- ProFlow™ XR 1500 bar Pump – Exceptional retention time precision and stability across the full low-flow range, reducing variability in complex separations.
- Low-Flow Split-Loop Autosampler – Limits sample dispersion, minimizes carryover, and optimizes sample usage for higher efficiency and sensitivity.
- Maximized Productivity: Fast, high-throughput analysis with reliable, low-maintenance operation and efficient MS data acquisition.
- Exceptional Performance: Accurate, reproducible results with minimal carryover and high sensitivity for low-abundance or complex samples.
- Seamless Workflow Integration: End-to-end Vanquish™ Neo UHPLC integration across nano-, capillary-, and micro-flow LC-MS (1 nL/min – 100 µL/min) with 24/7 robust performance.
- Reliable, Actionable Results: Consistent, high-quality LC–MS data for discovery, characterization, and regulatory studies.
LC-MS Analysis Capabilities & Applications
iFyber’s comprehensive LC-MS services - including E&L testing, metabolomics, proteomics, lipidomics, and more are designed around your specific research goals. We integrate method development, analysis, and interpretation to ensure the data you receive is directly relevant and actionable.
Our workflows directly support common challenges faced across industries:
- Pharma & Biotech: Lean teams, limited internal capacity, rapid iteration needs, and reliance on flexible, science-forward partners.
- Medical Device: Investigating unexpected findings, contamination events, or unknown extractables requiring rapid root-cause analysis.
- Academia: Untargeted, exploratory research without regulatory constraints and with a need for high-resolution, reproducible data.
Combined with our next-generation PODs engagement model, iFyber acts as an extension of your internal team, providing the expertise, capabilities, and scientific collaboration you need to tackle complex challenges efficiently.
LC-MS Analysis Services
Supporting regulatory submissions and product safety.
Extractables & Leachables (E&L) Testing
LC-MS enables precise identification and quantification of extractable and leachable compounds in medical devices, packaging, and container systems. Our team works with you to tailor each study to regulatory requirements and material profiles, delivering targeted, reliable data for premarket submissions or ongoing product safety monitoring.
For medical device and materials teams, LC-MS is especially powerful when unexpected anomalies arise — unexplained results, unusual profiles, odors, discoloration, or material incompatibilities. Our scientists specialize in forensic-level investigations to determine not only what is in the sample, but why it’s present and how it impacts product safety or performance.
Transforming how disease progression is understood.
Metabolomics for Wound & Disease State Analysis
Biological fluids contain thousands of metabolites. iFyber’s high-resolution LC-MS analysis methods profile and quantify these small molecules, supporting research into inflammation, chronic disease, and infection. From exploratory biomarker discovery to longitudinal studies, we utilize in-house LC-MS equipment to complete the in-depth analysis needed for innovative diagnostics and therapeutic development. Whether you’re identifying metabolic shifts or developing a new diagnostic pathway, we help turn raw data into actionable biological insight.
Because many early-phase therapeutic and diagnostic teams operate in unregulated discovery environments, we support rapid hypothesis generation, small-sample studies, and fast iterative cycles where biomarker exploration feeds directly into R&D decision-making.
Assess chemical and structural changes post sterilization or formulation.
Post-Sterilization & Processing Effects
What happens to peptides, proteins, or polymers after sterilization or other processing methods? Using LC-MS analytical methods, we can measure structural changes to provide detailed before-and-after molecular fingerprints to detect degradation, cross-linking, or unwanted modifications - providing insights that are vital to ensure product stability and performance validation. Ideal for R&D, product development, production, or manufacturing teams developing novel materials or biologic-device combinations.
Identify trace compounds in even the most complex matrices.
Environmental Testing for Contamination
From PFAS to unknown contaminants in water, food, or soil, we can develop and validate methods for difficult sample matrices. Combining LC-MS equipment with our team’s expertise, we support academic, government, or industrial partners in identifying unknowns and resolving what’s present at trace levels to deliver high-confidence data.
Our LC-MS analysis platform supports:
- Biopharma: High-resolution, accurate mass analysis of intact proteins (up to m/z 12,000) with HCD, ETD, and EThcD fragmentation for deep sequence coverage, PTM mapping, and impurity detection.
- Structural Biology: Residue-level HDX-MS enables precise protein conformational analysis, epitope mapping, and drug-binding site identification with fast, sensitive scans.
- Metabolomics: Enhanced dynamic range and electrodynamic ion funnel minimize unintended fragmentation, improving detection of low-abundance metabolites and annotation of unknowns.
- Proteomics: Accurate quantification, deep coverage, and PTM analysis using ETD/EThcD, supporting TMTpro 32plex and immunopeptidomics with maximized sequence coverage..
Why Partner with iFyber for LC-MS Testing
iFyber brings precision instrumentation, method development expertise, and collaborative problem-solving to every project. We’re more than a testing lab, we’re a research partner who can flex between high-throughput screening and deeply customized investigations.
Many of our partners come to us because they lack internal LC-MS capacity, face sample backlogs, or need untargeted insights beyond what routine labs can support. We help you understand what’s there and why it matters, enabling decisive action and confident next steps.
- Scientist-to-Scientist Collaboration: We don’t just hand over results, we interpret the “why” behind your data and help guide next steps.
- Rapid Turnaround, Reliable Reproducibility: Designed for early discovery and fast-moving R&D, our workflows emphasize both speed and rigor.
- Overflow Analytical Support: Extend your LC-MS capacity without expanding internal instrumentation or staffing.
- Flexible, Collaborative R&D Partner: Ideal for untargeted, exploratory, or mechanism-driven research where scientific agility matters more than compliance constraints.
- Multi-disciplinary integration with your research or product development goals.
Whether you need to screen hundreds of samples quickly or design a specialized study to uncover critical insights, iFyber delivers clarity, context, and confidence in every result