Liquid Chromatography Mass Spectrometry (LC-MS) Analysis
Metabolomics, Extractables and Leachables Testing, Environmental Contaminants, and Beyond
Liquid Chromatography–Mass Spectrometry (LC-MS) has revolutionized how researchers, product developers, and innovators answer the essential question: “What’s in this sample?”
At iFyber, our LC-MS analytical services go beyond basic data collection. We provide deep insight through custom method development, high-resolution analysis, and expert interpretation across complex biological, environmental, and material samples
What sets iFyber apart is the way we integrate LC-MS analysis with our broader R&D capabilities, including microbiology, molecular biology, polymer and biomaterial characterization, bioanalytical chemistry, and in vitro/ex vivo models. By combining liquid chromatography mass spectrometry with these complementary services, we design comprehensive studies that not only identify what’s present, but also explain its biological relevance, material performance, or regulatory impact.
Whether you’re developing medical devices, advancing therapeutics, or solving tough analytical challenges, iFyber’s LC-MS analytical services provide the clarity and context you need to move research forward with confidence
Our LC-MS Platform
iFyber’s LC–MS services are powered by the Orbitrap Excedion Pro hybrid quadrupole-Orbitrap mass spectrometer, a state-of-the-art system that delivers enhanced sensitivity, high resolution, and dynamic flexibility required for today’s complex analytical challenges.
This advanced platform combines high-resolution mass analysis, electron-based fragmentation, and gas-phase fractionation to capture molecular information with exceptional clarity. Designed for biopharma, metabolomics, proteomics, and structural biology applications, this platform sets a new standard in analytical performance, empowering groundbreaking discoveries.
Key features and Technology:
iFyber’s LC-MS platform combines cutting-edge mass spectrometry, ionization, and chromatography technologies to deliver high-resolution, high-sensitivity analysis for complex biological, environmental, and material samples.
By combining these technologies, iFyber delivers high-sensitivity liquid chromatography mass spectrometry analysis that supports both exploratory discovery and targeted quantification helping researchers and product developers uncover meaningful insights across biological, environmental, and material systems.
- Ultra-High-Field Orbitrap Mass Analyzer – Up to 70 Hz MS² scans for fast, precise data acquisition.
- Quadrupole & Ion-Routing Multipole – Enables versatile fragmentation strategies.
- EASY-IC & ETD – Accurate mass calibration and electron-based fragmentation for detailed molecular characterization.
- Bent Trap – High-duty cycles for enhanced sensitivity.
- FAIMS Pro Duo Interface – Online gas-phase fractionation reduces noise and improves system robustness.
- OptaMax™ Plus Ion Source & EasySpray™ – Ideal technolgy for high-sensitivity LC-MS data.
- Seamless Thermo Scientific™ PepMap™ Neo Column Integration – tool-free setup, superior separation performance, and column-to-column reproducibility, enhancing overall workflow flexibility.
- ProFlow™ XR 1500 bar Pump – Exceptional retention time precision and stability across the full low-flow range, reducing variability in complex separations.
- Low-Flow Split-Loop Autosampler – Limits sample dispersion, minimizes carryover, and optimizes sample usage for higher efficiency and sensitivity.
- Maximized Productivity: Fast, high-throughput analysis with reliable, low-maintenance operation and efficient MS data acquisition.
- Exceptional Performance: Accurate, reproducible results with minimal carryover and high sensitivity for low-abundance or complex samples.
- Seamless Workflow Integration: End-to-end Vanquish™ Neo UHPLC integration across nano-, capillary-, and micro-flow LC-MS (1 nL/min – 100 µL/min) with 24/7 robust performance.
- Reliable, Actionable Results: Consistent, high-quality LC–MS data for discovery, characterization, and regulatory studies.
LC-MS Capabilities & Applications
iFyber’s comprehensive LC-MS services - including extractables and leachables (E&L) testing, metabolomics, proteomics, lipidomics, and more – are designed around your specific research goals. Offering both high-throughput work and custom studies, we integrate method development, analysis, and interpretation to ensure the data you receive is directly relevant and actionable. Combined with our next-generation PODs engagement model, iFyber acts as an extension of your internal team, providing the expertise, capabilities, and resources you need to tackle complex challenges efficiently.
LC-MS Analytical Services
Supporting regulatory submissions and product safety.
Extractables & Leachables (E&L) Testing
LC-MS enables precise identification and quantification of extractable and leachable compounds in medical devices, packaging, and container systems. Our team works with you to tailor each study to regulatory requirements and material profiles, delivering targeted, reliable data for premarket submissions or ongoing product safety monitoring.
Transforming how disease progression is understood.
Metabolomics for Wound & Disease State Analysis
Biological fluids contain thousands of metabolites. iFyber’s high-resolution LC-MS analytical methods profile and quantify these small molecules, supporting research into inflammation, chronic disease, and infection. From exploratory biomarker discovery to longitudinal studies, we utilize cutting-edge LC-MS equipment to complete the in-depth analysis needed for innovative diagnostics and therapeutic development. Whether you’re identifying metabolic shifts or developing a new diagnostic pathway, we help turn raw data into actionable biological insight.
Assess chemical and structural changes post sterilization or formulation.
Post-Sterilization & Processing Effects
What happens to peptides, proteins, or polymers after sterilization or other processing methods? Using LC-MS analytical methods, we can measure structural changes to provide detailed before-and-after molecular fingerprints to detect degradation, cross-linking, or unwanted modifications - providing insights that are vital to ensure product stability and performance validation. Ideal for R&D, product development, production, or manufacturing teams developing novel materials or biologic-device combinations.
Identify trace compounds in even the most complex matrices.
Environmental Testing for Contamination
From PFAS to unknown contaminants in water, food, or soil, we can develop and validate methods for difficult sample matrices. Combining LC-MS equipment with our team’s expertise, we support academic, government, or industrial partners in identifying unknowns and resolving what’s present at trace levels to deliver high-confidence data.
Our LC-MS analysis platform supports:
- Biopharma: High-resolution, accurate mass analysis of intact proteins (up to m/z 12,000) with HCD, ETD, and EThcD fragmentation for deep sequence coverage, PTM mapping, and impurity detection.
- Structural Biology: Residue-level HDX-MS enables precise protein conformational analysis, epitope mapping, and drug-binding site identification with fast, sensitive scans.
- Metabolomics: Enhanced dynamic range and electrodynamic ion funnel minimize unintended fragmentation, improving detection of low-abundance metabolites and annotation of unknowns.
- Proteomics: Accurate quantification, deep coverage, and PTM analysis using ETD/EThcD, supporting TMTpro 32plex and immunopeptidomics with maximized sequence coverage..
Partner with iFyber for LC-MS Testing
iFyber brings precision instrumentation, method development expertise, and collaborative problem-solving to every project. We’re more than a testing lab — we’re a research partner who can flex between high-throughput screening and deeply customized investigations. Our liquid chromatography mass spectrometry services are designed to answer questions that fall outside the expertise of routine testing laboratories. Custom method development, targeted searches, complicated extractions are all part of our bespoke LC-MS offering.
- High-resolution analysis across hundreds of analytes
- Custom method development for novel targets
- Interpretive insight beyond the numbers
- Extension of your team offering specialized expertise/resources and high-touch support
- Collaborative conversations with your team to design LC-MS studies aligned with your scientific objectives
- Flexible project sizes from quick metabolite detection to complicated proteomics analysis
- Multi-disciplinary integration with your research or product development goals
Whether you need to screen hundreds of samples quickly or design a specialized study to uncover critical insights, iFyber delivers clarity, context, and confidence in every result.