Antimicrobial / Anti-biofilm

iFyber provides a range of in vitro and ex vivo assays for both planktonic bacteria (free-floating) and biofilms (complex microbial communities) that can be tailored to test technologies in a way that meets the client’s objective.

We focus on determining the efficacy of new technologies and actively develop novel assay systems based on our client’s specific needs. Initial in vitro test methods can be used to screen formulations and measure antimicrobial and anti-biofilm efficacy for a range of applications, from therapeutics to antifouling technologies. For wound care applications, our ex vivo model allows for the evaluation of prototype formulations on a more clinically relevant matrix before the start of costly in vivo animal studies – saving money and reducing time to market.

Examples of relevant materials for testing include new antibiotics/biocides, coatings, nanomaterials, wound dressings, coated natural tissue matrices, and coated medical devices.

Antimicrobial Susceptibility Testing

  • Dynamic contact assay
  • Time-kill assay
  • Zone of inhibition assay
  • Time course of antimicrobial activity
  • MIC assay
  • MTT assay
  • Bacterial selective plating
  • Bacterial attachment

Anti-Biofilm Efficacy Testing

  • MBEC in vitro assay
  • Microwell biofilm susceptibility and eradication assays
  • Porcine dermal explant ex vivo model
  • Artificial sputum in vitro model of cystic fibrosis

Histology & Imaging

  • Simple staining for bacteria (e.g., methylene blue, safranin, crystal violet)
  • Differential staining (e.g., Gram staining, GMS staining)
  • LIVE/DEAD staining
  • Confocal microscopy and 3D reconstruction for ex vivo model
    • Visualization of bacteria using GFP or luciferase (lux) reporter systems

Contact us today to discuss your application-specific antimicrobial or anti-biofilm testing needs.